Rabbit monoclonal antibody raised against native human IgA1.
Native purified IgA from human plasma.
This antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Protein A affinity purification
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture) Flow Cytometry Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (0.1-1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil tissue with Human IgA1 monoclonal antibody, clone RM124 (Cat # MAB12814) under 1 ug/mL working concentration.
ELISA analysis of Human IgA1 monoclonal antibody, clone RM124 (Cat # MAB12814) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both human IgA1 and IgA2; no cross reactivity human IgG, IgM, IgD, or IgE. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.