Human IgA monoclonal antibody, clone RM128
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgA monoclonal antibody, clone RM128 (Cat # MAB12813) under 1 ug/mL working concentration.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgA monoclonal antibody, clone RM128 (Cat # MAB12813) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both human IgA1 and IgA2; no cross reactivity human IgG, IgM, IgD, or IgE. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgA monoclonal antibody, clone RM128 (Cat# MAB12813). The plate was coated with different amounts of human IgA. A serial dilution of Cat# MAB12813 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgA monoclonal antibody, clone RM128 (Cat# MAB12813) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgA monoclonal antibody, clone RM128 (Cat# MAB12813) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IgA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human IgA.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymphoid tissue with Human IgA monoclonal antibody, clone RM128 (Cat # MAB12813) under 1 ug/mL working concentration.Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgA monoclonal antibody, clone RM128 (Cat # MAB12813) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both human IgA1 and IgA2; no cross reactivity human IgG, IgM, IgD, or IgE. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgA monoclonal antibody, clone RM128 (Cat# MAB12813). The plate was coated with different amounts of human IgA. A serial dilution of Cat# MAB12813 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgA monoclonal antibody, clone RM128 (Cat# MAB12813) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA using Human IgA monoclonal antibody, clone RM128 (Cat# MAB12813) as the capture antibody (100ng/well), and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Flow Cytometry
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Gene Info — IGHA1
Entrez GeneID
3493Protein Accession#
P01876;P01877Gene Name
IGHA1
Gene Alias
FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, IgA1, MGC102857
Gene Description
immunoglobulin heavy constant alpha 1
Omim ID
146900Gene Ontology
HyperlinkOther Designations
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Gene Info — IGHA2
Entrez GeneID
3494Protein Accession#
P01876;P01877Gene Name
IGHA2
Gene Alias
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Gene Description
immunoglobulin heavy constant alpha 2 (A2m marker)
Omim ID
147000Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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