Human Ig light chain monoclonal antibody, clone RM129 (Biotin)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Detection of human IgG1 in monkey serum, using Cat# MAB12810 (capture) and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
Enzyme-linked Immunoabsorbent Assay
ELISA showing Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12811 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat # MAB12811) at the following concentrations: 0.1 ug/mL, 0.02 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both kappa and lambda light chains of human immunoglobulins; no cross reactivity with mouse, rat, or goat immunoglobulin light chain. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer Sandwich ELISA using Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody. The plate, coated with the Cat# MAB12811, was loaded with different amounts of human IgG. A serial dilution of Cat# MAB12811 was used as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human Ig light chain.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human IgG.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain.
Form
Liquid
Conjugation
Biotin
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.02-0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Immunohistochemistry
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Detection of human IgG1 in monkey serum, using Cat# MAB12810 (capture) and Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.Enzyme-linked Immunoabsorbent Assay
ELISA showing Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Cat# MAB12811 and a monkey IgG binding antibody (positive control) was used as the detection antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat # MAB12811) at the following concentrations: 0.1 ug/mL, 0.02 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both kappa and lambda light chains of human immunoglobulins; no cross reactivity with mouse, rat, or goat immunoglobulin light chain. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer Sandwich ELISA using Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat# MAB12811) as the detection antibody. The plate, coated with the Cat# MAB12811, was loaded with different amounts of human IgG. A serial dilution of Cat# MAB12811 was used as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Flow Cytometry
-
Gene Info — IGKC
-
Gene Info — IGLC1
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com