Human IgG (gamma heavy chain) monoclonal antibody, clone RM116
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil tissue with Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) under 1 ug/mL working concentration.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat# MAB12808). The plate was coated with different amounts of human IgG1. A serial dilution of Cat# MAB12808 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the gamma 1, gamma 2, gamma3, gamma 4 of human IgGs and the Fc of human IgG; no cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IgG (gamma heavy chain).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human IgG.
Sequence
N/A
Reactivity
Human
Specificity
This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including gamma 1, gamma2, gamma 3, and gamma 4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil tissue with Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) under 1 ug/mL working concentration.Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
A titer ELISA using Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat# MAB12808). The plate was coated with different amounts of human IgG1. A serial dilution of Cat# MAB12808 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the gamma 1, gamma 2, gamma3, gamma 4 of human IgGs and the Fc of human IgG; no cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — IGHG1
Entrez GeneID
3500Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG1
Gene Alias
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Gene Description
immunoglobulin heavy constant gamma 1 (G1m marker)
Omim ID
147100Gene Ontology
HyperlinkOther Designations
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Gene Info — IGHG2
Entrez GeneID
3501Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG2
Gene Alias
DKFZp686I04196
Gene Description
immunoglobulin heavy constant gamma 2 (G2m marker)
Omim ID
147110Gene Ontology
HyperlinkOther Designations
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Gene Info — IGHG3
Entrez GeneID
3502Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG3
Gene Alias
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, MGC45809
Gene Description
immunoglobulin heavy constant gamma 3 (G3m marker)
Omim ID
147120Gene Ontology
HyperlinkOther Designations
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Gene Info — IGHG4
Entrez GeneID
3503Protein Accession#
P01857;P01859;P01860;P01861Gene Name
IGHG4
Gene Alias
MGC117419
Gene Description
immunoglobulin heavy constant gamma 4 (G4m marker)
Omim ID
147130Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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