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Human IgG (gamma heavy chain) monoclonal antibody, clone RM116

  • Catalog # : MAB12808
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against native human IgG (gamma heavy chain).
  • Immunogen:
  • Native purified IgG from human plasma.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including gamma 1, gamma2, gamma 3, and gamma 4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil tissue with Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) under 1 ug/mL working concentration.
  • Immunocytochemistry
  • ELISA
  • ELISA
  • ELISA analysis of Human IgG (gamma heavy chain) monoclonal antibody, clone RM116 (Cat # MAB12808) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the gamma 1, gamma 2, gamma3, gamma 4 of human IgGs and the Fc of human IgG; no cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 3500
  • Protein Accession#:
  • P01857;P01859;P01860;P01861
  • Gene Name:
  • IGHG1
  • Gene Alias:
  • -
  • Gene Description:
  • immunoglobulin heavy constant gamma 1 (G1m marker)
  • Other Designations:
  • -
  • Gene Information
  • Entrez GeneID:
  • 3501
  • Protein Accession#:
  • P01857;P01859;P01860;P01861
  • Gene Name:
  • IGHG2
  • Gene Alias:
  • DKFZp686I04196
  • Gene Description:
  • immunoglobulin heavy constant gamma 2 (G2m marker)
  • Other Designations:
  • -
  • Gene Information
  • Entrez GeneID:
  • 3502
  • Protein Accession#:
  • P01857;P01859;P01860;P01861
  • Gene Name:
  • IGHG3
  • Gene Alias:
  • DKFZp686H11213,FLJ39988,FLJ40036,FLJ40253,FLJ40587,FLJ40789,FLJ40834,IgG3,MGC45809
  • Gene Description:
  • immunoglobulin heavy constant gamma 3 (G3m marker)
  • Other Designations:
  • -
  • Gene Information
  • Entrez GeneID:
  • 3503
  • Protein Accession#:
  • P01857;P01859;P01860;P01861
  • Gene Name:
  • IGHG4
  • Gene Alias:
  • MGC117419
  • Gene Description:
  • immunoglobulin heavy constant gamma 4 (G4m marker)
  • Other Designations:
  • -
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