Product Browser

Last updated: 2017/11/19

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

His-Tag monoclonal antibody, clone RM146

  • Catalog # : MAB12807
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of His-Tag.
  • Immunogen:
  • A mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to recombinant proteins containing the 6X His-Tag or 10X His-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (0.5 ug/mL-2 ug/mL)
    Immunocytochemistry (0.5 ug/mL-2 ug/mL)
    Immunohistochemistry (0.1 ug/mL-1 ug/mL)
    Immunoprecipitation (0.5 ug/mL-2 ug/mL)
    Western Blot (0.1 ug/mL-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of 293T cell Lane 1: untransfected with His-Tag fusion protein X, Lane 2: transfected with His-Tag fusion protein X, Lane 3: E. coli lysate without His-Tag Protein Y and Lane 4: E. coli lysate with His-Tag Protein Y with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807) at 0.2 ug/mL working concentration.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807).
  • Immunocytochemistry
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation analysis of 293T cells expressing a His-Tag fusion protein with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807). Lane 1: whole lysate (Control), Lane 2: IP by rabbit IgG (Control) and Lane 3: IP by His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807).
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Flow Cytometry
  • RSS
  • YouTube
  • Linkedin
  • Facebook