His-Tag monoclonal antibody, clone RM146
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More Files
- More Functions
Western Blot
Western blot analysis of 293T cell Lane 1: untransfected with His-Tag fusion protein X, Lane 2: transfected with His-Tag fusion protein X, Lane 3: E. coli lysate without His-Tag Protein Y and Lane 4: E. coli lysate with His-Tag Protein Y with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807) at 0.2 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807).
Immunoprecipitation
Immunoprecipitation analysis of 293T cells expressing a His-Tag fusion protein with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807). Lane 1: whole lysate (Control), Lane 2: IP by rabbit IgG (Control) and Lane 3: IP by His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of His-Tag.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.
Sequence
N/A
Specificity
This antibody reacts to recombinant proteins containing the 6X His-Tag or 10X His-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (0.5 ug/mL-2 ug/mL)
Immunocytochemistry (0.5 ug/mL-2 ug/mL)
Immunohistochemistry (0.1 ug/mL-1 ug/mL)
Immunoprecipitation (0.5 ug/mL-2 ug/mL)
Western Blot (0.1 ug/mL-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of 293T cell Lane 1: untransfected with His-Tag fusion protein X, Lane 2: transfected with His-Tag fusion protein X, Lane 3: E. coli lysate without His-Tag Protein Y and Lane 4: E. coli lysate with His-Tag Protein Y with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807) at 0.2 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807).Immunocytochemistry
Immunoprecipitation
Immunoprecipitation analysis of 293T cells expressing a His-Tag fusion protein with His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807). Lane 1: whole lysate (Control), Lane 2: IP by rabbit IgG (Control) and Lane 3: IP by His-Tag monoclonal antibody, clone RM146 (Cat # MAB12807).Flow Cytometry
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