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Histone H4 monoclonal antibody, clone RM212

  • Catalog # : MAB12806
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human Histone H4.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus region of Histone H4.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.2 ug/mL-1 ug/mL)
    Western Blot (0.1 ug/mL-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: A375, Lane 2: HEK293, Lane 3: HeLa and Lane 4: SK-MEL-2 whole cell lysate with Histone H4 monoclonal antibody, clone RM212 (Cat # MAB12806) at 0.2 ug/mL working concentration, showed endogenous Histone H4 in A375, HEK293, HeLa and SK-MEL-2 cells.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • HIST4H4
  • Gene Alias:
  • H4/p,MGC24116
  • Gene Description:
  • histone cluster 4, H4
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq
  • Other Designations:
  • histone 4, H4,histone H4
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