Pan Histone H3 monoclonal antibody, clone RM188
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: recombinant Histone H3.3, Lane 2: recombinant Histone H3.1, Lane 3: A375, Lane 4: HEK293, Lane 5: HeLa and Lane 6: SK-MEL-2 whole cell lysate with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) at 0.025 ug/mL working concentration, showed a band of recombinant Histone H3.3, recombinant Histone H3.1 and total Histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
Sandwich ELISA
Sandwich ELISA analysis of HeLa whole cell lysate treated or untreated with Sodium Butyrate (NaBu) using Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804). A: Using Pan Histone H3 monoclonal antibody, clone RM188 as the capture antibody at 1 ug/mL working concentration and biotinylated format of Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) as the detection antibody at 1 ug/mL working concentration. B: Using Histone H3 (acetyl K79) monoclonal antibody, clone RM156 as the capture antibody at 5 ug/mL working concentration and biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12837) as the detection antibody at 1 ug/mL working concentration.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Pan Histone H3 monoclonal antibody, clone RM188 (Cat# MAB12804) reacts to both Histone H3.1 and Histone H3.3.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human Pan histone H3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus region of human Pan Histone H3.
Sequence
N/A
Specificity
This antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Sandwich ELISA (0.2 ug/mL-1 ug/mL)
Immunocytochemistry (1 ug/mL-5 ug/mL)
Western Blot (0.01 ug/mL-0.25 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: recombinant Histone H3.3, Lane 2: recombinant Histone H3.1, Lane 3: A375, Lane 4: HEK293, Lane 5: HeLa and Lane 6: SK-MEL-2 whole cell lysate with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) at 0.025 ug/mL working concentration, showed a band of recombinant Histone H3.3, recombinant Histone H3.1 and total Histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.Immunocytochemistry
Immunocytochemical staining of HeLa cells with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).Sandwich ELISA
Sandwich ELISA analysis of HeLa whole cell lysate treated or untreated with Sodium Butyrate (NaBu) using Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804). A: Using Pan Histone H3 monoclonal antibody, clone RM188 as the capture antibody at 1 ug/mL working concentration and biotinylated format of Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) as the detection antibody at 1 ug/mL working concentration. B: Using Histone H3 (acetyl K79) monoclonal antibody, clone RM156 as the capture antibody at 5 ug/mL working concentration and biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12837) as the detection antibody at 1 ug/mL working concentration. -
Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P84243Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
-
Interactome
-
Pathway
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com