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Last updated: 2017/3/26

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Pan Histone H3 monoclonal antibody, clone RM188

  • Catalog # : MAB12804
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human Pan Histone H3.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus region of Pan Histone H3.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Sandwich ELISA (0.2 ug/mL-1 ug/mL)
    Immunocytochemistry (1 ug/mL-5 ug/mL)
    Western Blot (0.01 ug/mL-0.25 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: recombinant Histone H3.3, Lane 2: recombinant Histone H3.1, Lane 3: A375, Lane 4: HEK293, Lane 5: HeLa and Lane 6: SK-MEL-2 whole cell lysate with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) at 0.025 ug/mL working concentration, showed a band of recombinant Histone H3.3, recombinant Histone H3.1 and total Histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of HeLa cells with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
  • Sandwich ELISA
  • Sandwich ELISA
  • Sandwich ELISA analysis of HeLa whole cell lysate treated or untreated with Sodium Butyrate (NaBu) using Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804). A: Using Pan Histone H3 monoclonal antibody, clone RM188 as the capture antibody at 1 ug/mL working concentration and biotinylated format of Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) as the detection antibody at 1 ug/mL working concentration. B: Using Histone H3 (acetyl K79) monoclonal antibody, clone RM156 as the capture antibody at 5 ug/mL working concentration and biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12837) as the detection antibody at 1 ug/mL working concentration.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 8350
  • Gene Name:
  • HIST1H3A
  • Gene Alias:
  • H3/A,H3FA
  • Gene Description:
  • histone cluster 1, H3a
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member A,histone 1, H3a
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