Rabbit monoclonal antibody raised against synthetic peptide of human Histone H2AZ.
Immunogen:
A synthetic peptide corresponding to C-terminus region of Histone H2AZ.
Host:
Rabbit
Specificity:
This antibody reacts to Histone H2AZ protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Form:
Liquid
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
ELISA (0.1 - 1 ug/mL) Immunocytochemistry (1 - 2 ug/mL) Western Blot (0.1 - 0.5 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: A375, Lane 2: HEK293, Lane 3: HeLa and Lane 4: SK-MEL-2 whole cell lysate with Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) at 0.5 ug/mL working concentration, showed endogenous Histone H2AZ in A375, HEK293, HeLa and SK-MEL-2 cells.
Immunocytochemistry
Immunocytochemistry of HeLa cells, using Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq