Histone H2AZ monoclonal antibody, clone RM215
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: A375, Lane 2: HEK293, Lane 3: HeLa and Lane 4: SK-MEL-2 whole cell lysate with Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) at 0.5 ug/mL working concentration, showed endogenous Histone H2AZ in A375, HEK293, HeLa and SK-MEL-2 cells.
Immunocytochemistry
Immunocytochemistry of HeLa cells, using Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H2AZ.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus region of human Histone H2AZ.
Sequence
N/A
Specificity
This antibody reacts to Histone H2AZ protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.1 - 1 ug/mL)
Immunocytochemistry (1 - 2 ug/mL)
Western Blot (0.1 - 0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: A375, Lane 2: HEK293, Lane 3: HeLa and Lane 4: SK-MEL-2 whole cell lysate with Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) at 0.5 ug/mL working concentration, showed endogenous Histone H2AZ in A375, HEK293, HeLa and SK-MEL-2 cells.Immunocytochemistry
Immunocytochemistry of HeLa cells, using Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) (red). Actin filaments have been labeled with fluorescein phalloidin (green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — H2AFZ
Entrez GeneID
3015Protein Accession#
P0C0S5Gene Name
H2AFZ
Gene Alias
H2A.z, H2A/z, H2AZ, MGC117173
Gene Description
H2A histone family, member Z
Omim ID
142763Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq
Other Designations
H2AZ histone
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Interactome
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Pathway
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