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Last updated: 2022/5/22

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Histone H2AZ monoclonal antibody, clone RM215 

  • Catalog # : MAB12803
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human Histone H2AZ.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus region of Histone H2AZ.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to Histone H2AZ protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.1 - 1 ug/mL)
    Immunocytochemistry (1 - 2 ug/mL)
    Western Blot (0.1 - 0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: A375, Lane 2: HEK293, Lane 3: HeLa and Lane 4: SK-MEL-2 whole cell lysate with Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) at 0.5 ug/mL working concentration, showed endogenous Histone H2AZ in A375, HEK293, HeLa and SK-MEL-2 cells.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry of HeLa cells, using Histone H2AZ monoclonal antibody, clone RM215 (Cat # MAB12803) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 3015
  • Gene Name:
  • H2AFZ
  • Gene Alias:
  • H2A.z,H2A/z,H2AZ,MGC117173
  • Gene Description:
  • H2A histone family, member Z
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq
  • Other Designations:
  • H2AZ histone
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