Rabbit monoclonal antibody raised against synthetic phosphopeptide of human Histone H3 (S10).
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S10 of Histone H3.
Host:
Rabbit
Specificity:
This antibody reacts to Histone H3 phosphorylated at Serine 10. No cross reactivity with other phosphorylated histones.
Form:
Liquid
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
ELISA (0.2 ug/mL-1 ug/mL) Immunocytochemistry (0.5 ug/mL-2 ug/mL) Western Blot (0.5 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with Nocodazole and Lane 2: acid extracts of HeLa cell untreated with Histone H3 (phospho S10) monoclonal antibody, clone RM163 (Cat # MAB12793) at 0.5 ug/mL working concentration, showed a band of Histone H3 phosphorylated at Serine 10.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with Histone H3 (phospho S10) monoclonal antibody, clone RM163 (Cat # MAB12793) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq