Histone H2A/H4 (phospho S1) monoclonal antibody, clone RM216
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More Files
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Western Blot (Cell lysate)
Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with Nocodazole and Lane 2: acid extracts of HeLa cell untreated with Histone H2A/H4 (phospho S1) monoclonal antibody, clone RM216 (Cat # MAB12792) at 0.5 ug/mL working concentration, showed a band of Histone H2A and H4 phosphorylated at Serine 1.
Immunocytochemistry
Immunocytochemistry of HeLa cells, using Histone H2A/H4 (phospho S1) monoclonal antibody, clone RM216 (Cat# MAB12792) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H2A/H4 (phospho S1) monoclonal antibody, clone RM216 (Cat# MAB12792) specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H2A/H4 (S1).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S1 of human Histone H2A.
Sequence
N/A
Specificity
This antibody reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.2 ug/mL-1 ug/mL)
Western Blot (0.5 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: acid extracts of HeLa cell treated with Nocodazole and Lane 2: acid extracts of HeLa cell untreated with Histone H2A/H4 (phospho S1) monoclonal antibody, clone RM216 (Cat # MAB12792) at 0.5 ug/mL working concentration, showed a band of Histone H2A and H4 phosphorylated at Serine 1.Immunocytochemistry
Immunocytochemistry of HeLa cells, using Histone H2A/H4 (phospho S1) monoclonal antibody, clone RM216 (Cat# MAB12792) (red). Actin filaments have been labeled with fluorescein phalloidin (green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST1H2AE
Entrez GeneID
3012Protein Accession#
P16104;P62805Gene Name
HIST1H2AE
Gene Alias
H2A.1, H2A.2, H2A/a, H2AFA
Gene Description
histone cluster 1, H2ae
Omim ID
602786Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H2A histone family, member A|histone 1, H2ae|histone H2AE
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Gene Info — HIST4H4
Entrez GeneID
121504Protein Accession#
P16104;P62805Gene Name
HIST4H4
Gene Alias
H4/p, MGC24116
Gene Description
histone cluster 4, H4
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq
Other Designations
histone 4, H4|histone H4
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