Western blot analysis of Lane 1: acid extracts of HeLa cell untreated, Lane 2: acid extracts of HeLa cell treated with sodium butyrate and Lane 3: recombinant Histone H3.3 with Histone H3 (acetyl K23) monoclonal antibody, clone RM169 (Cat # MAB12790) at 1 ug/mL working concentration, showed a band of Histone H3 acetylated at Lysine 23.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq