Rabbit monoclonal antibody raised against synthetic peptide of human Histone H3 (K4).
A synthetic peptide corresponding to residues surrounding K4 at N-terminus region of Histone H3.
This antibody recognizes Histone H3 that is unmodified at Lys 4 but does not recognize acetylated or methylated Lys 4. The antibody binding specificity allows for modifications of Arg 2, Thr 3, and/or other modifications in Histone H3.
Protein A purification
ELISA (0.5 ug/mL-1 ug/mL) Western Blot (1 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: acid extracts of HeLa cell treated with sodium butyrate (HeLa+NaBu), Lane 2: acid extracts of HeLa cell treated with Nocodazole (HeLa+Noc), Lane 3: acid extracts of HeLa cell untreated and Lane 4: recombinant Histone H3.1 with Histone H3 (K4) monoclonal antibody, clone RM186 (Cat # MAB12789) at 1 ug/mL working concentration, showed a band of Histone H3 with unmodified Lysine 4.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq