Western blot analysis of Lane 1: acid extracts of HeLa cell treated with sodium butyrate, Lane 2: acid extracts of HeLa cell untreated and Lane 3: recombinant Histone H4 with Histone H4 (acetyl K8) monoclonal antibody, clone RM201 (Cat # MAB12786) at 0.5 ug/mL working concentration, showed a band of Histone H4 acetylated at Lysine 8.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq