Rabbit monoclonal antibody raised against synthetic monomethyl peptide of human Histone H4 (monomethyl R3).
A synthetic monomethyl peptide corresponding to residues surrounding R3 of Histone H4.
This antibody reacts to Histone H4 monomethylated at Arginine 3. No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
Protein A purification
ELISA (0.2 ug/mL-1 ug/mL) Western Blot (0.5 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: acid extracts of HeLa cell and Lane 2: recombinant Histone H4 with Histone H4 (monomethyl R3) monoclonal antibody, clone RM195 (Cat # MAB12784) at 0.5 ug/mL working concentration, showed a band of Histone H4 monomethylated at Arginine 3.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq