Histone H4 (phospho S1) monoclonal antibody, clone RM194
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More Files
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Western Blot
Western blot analysis of Lane 1: acid extracts of HeLa cell and Lane 2: recombinant Histone H4 with Histone H4 (phospho S1) monoclonal antibody, clone RM194 (Cat # MAB12783) at 0.5 ug/mL working concentration, showed a band of Histone H4 phosphorylated at Serine 1.
Immunocytochemistry
Immunocytochemistry of HeLa cells, using Histone H4 (phospho S1) monoclonal antibody, clone RM194 (Cat# MAB12783) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H4 (phospho S1) monoclonal antibody, clone RM194 (Cat# MAB12783) specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H4 (S1).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S1 of human Histone H4.
Sequence
N/A
Specificity
This antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross reactivity with Histone H2A phosphorylated at Serine 1.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.2 ug/mL-1 ug/mL)
Western Blot (0.5 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: acid extracts of HeLa cell and Lane 2: recombinant Histone H4 with Histone H4 (phospho S1) monoclonal antibody, clone RM194 (Cat # MAB12783) at 0.5 ug/mL working concentration, showed a band of Histone H4 phosphorylated at Serine 1.Immunocytochemistry
Immunocytochemistry of HeLa cells, using Histone H4 (phospho S1) monoclonal antibody, clone RM194 (Cat# MAB12783) (red). Actin filaments have been labeled with fluorescein phalloidin (green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST4H4
Entrez GeneID
121504Protein Accession#
P62805Gene Name
HIST4H4
Gene Alias
H4/p, MGC24116
Gene Description
histone cluster 4, H4
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq
Other Designations
histone 4, H4|histone H4
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Interactome
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Pathway
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