Western blot analysis of Lane 1: recombinant Histone H3.3 and Lane 2: acid extracts of HeLa cell with Histone H3 (dimethyl K23) monoclonal antibody, clone RM171 (Cat # MAB12780) at 0.5 ug/mL working concentration, showed a band of Histone H3 dimethylated at Lysine 23.
Dot Blot (Peptide)
Dot Blot (Peptide) analysis of Histone H3 (dimethyl K23) monoclonal antibody, clone RM171 (Cat # MAB12780) only reacted to Histone H3 dimethyl-Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23 (K23Ctrl), monomethylated Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq