Western blot analysis of Lane 1: recombinant Histone H3.3 and Lane 2: acid extracts of HeLa cell with Histone H3 (dimethyl K18) monoclonal antibody, clone RM168 (Cat # MAB12778) at 0.5 ug/mL working concentration, showed a band of Histone H3 dimethylated at Lysine 18.
Dot Blot (Peptide)
Dot Blot (Peptide) analysis of Histone H3 (dimethyl K18) monoclonal antibody, clone RM168 (Cat # MAB12778) only reacted to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq