Histone H3 (dimethyl K18) monoclonal antibody, clone RM168
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: recombinant Histone H3.3 and Lane 2: acid extracts of HeLa cell with Histone H3 (dimethyl K18) monoclonal antibody, clone RM168 (Cat # MAB12778) at 0.5 ug/mL working concentration, showed a band of Histone H3 dimethylated at Lysine 18.
Dot Blot (Peptide)
Dot Blot (Peptide) analysis of Histone H3 (dimethyl K18) monoclonal antibody, clone RM168 (Cat # MAB12778) only reacted to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H3 (dimethyl K18) monoclonal antibody, clone RM168 (Cat# MAB12778) specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H3 (dimethyl K18).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic dimethyl peptide corresponding to residues surrounding K18 of human Histone H3.
Sequence
N/A
Specificity
This antibody reacts to Histone H3 dimethylated at Lysine 18. No cross reactivity with monomethylated Lysine 18 or trimethylated Lysine 18, or other methylation in histone H3.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Dot Blot
ELISA (0.1 ug/mL-0.5 ug/mL)
Western Blot (0.2 ug/mL-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: recombinant Histone H3.3 and Lane 2: acid extracts of HeLa cell with Histone H3 (dimethyl K18) monoclonal antibody, clone RM168 (Cat # MAB12778) at 0.5 ug/mL working concentration, showed a band of Histone H3 dimethylated at Lysine 18.Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot (Peptide) analysis of Histone H3 (dimethyl K18) monoclonal antibody, clone RM168 (Cat # MAB12778) only reacted to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2). -
Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P84243Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Pathway
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