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Mouse IgG Fc monoclonal antibody, clone RMG06

  • Catalog # : MAB12776
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  • Specification
  • Product Description:
  • Goat monoclonal antibody raised against full length native mouse IgG Fc.
  • Immunogen:
  • Native purified mouse IgG.
  • Host:
  • Goat
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody reacts to the Fc region of Mouse IgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.05 ug/mL-1 ug/mL)
    Flow Cytometry
    Immunocytochemistry
    Immunohistochemistry (0.5 ug/mL-2 ug/mL)
    Immunoprecipitation
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of Mouse IgG Fc monoclonal antibody, clone RMG06 (Cat # MAB12776) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow Cytometry
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