Mouse IgG Fc monoclonal antibody, clone RMG06
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG Fc monoclonal antibody, clone RMG06 (Cat # MAB12776) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of Mouse IgG Fc monoclonal antibody, clone RMG06 (Cat# MAB12776) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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Specification
Product Description
Goat recombinant monoclonal antibody raised against mouse IgG Fc.
Antibody Species
Goat
Immunogen
Original antibody is raised against mouse IgG.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to the Fc region of Mouse IgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.
Form
Liquid
Purification
Protein G purification
Isotype
IgG
Recommend Usage
ELISA (0.05 ug/mL-1 ug/mL)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (0.5 ug/mL-2 ug/mL)
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG Fc monoclonal antibody, clone RMG06 (Cat # MAB12776) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of Mouse IgG Fc monoclonal antibody, clone RMG06 (Cat# MAB12776) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.Flow Cytometry
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