Goat monoclonal antibody raised against full length native rabbit IgG Fc.
Native purified rabbit IgG.
This antibody reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
Protein G purification
ELISA (0.01 ug/mL- 0.5 ug/mL) Flow Cytometry Immunocytochemistry (0.5 ug/mL-2 ug/mL) Immunohistochemistry (0.5 ug/mL-2 ug/mL) Immunoprecipitation Western Blot (0.1 ug/mL-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).
ELISA analysis of IgG from different species with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.