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Rabbit IgG Fc monoclonal antibody, clone RMG02

  • Catalog # : MAB12774
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  • Specification
  • Product Description:
  • Goat monoclonal antibody raised against full length native rabbit IgG Fc.
  • Immunogen:
  • Native purified rabbit IgG.
  • Host:
  • Goat
  • Reactivity:
  • Rabbit
  • Specificity:
  • This antibody reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.01 ug/mL- 0.5 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5 ug/mL-2 ug/mL)
    Immunohistochemistry (0.5 ug/mL-2 ug/mL)
    Immunoprecipitation
    Western Blot (0.1 ug/mL-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of IgG from different species with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow Cytometry
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