Goat monoclonal antibody raised against full length native rabbit IgG Fab.
Native purified rabbit IgG.
This antibody reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG
Protein G purification
ELISA (0.01 ug/mL- 0.5 ug/mL) Flow Cytometry Immunocytochemistry (0.5 ug/mL-2 ug/mL) Immunohistochemistry (0.5 ug/mL-2 ug/mL) Immunoprecipitation Western Blot (0.1 ug/mL-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Cat # MAB12773) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).
ELISA analysis of IgG from different species with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Cat # MAB12773) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.