Mouse IgG3 monoclonal antibody, clone RM218
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG3 monoclonal antibody, clone RM218 (Cat # MAB12770) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both mouse IgG3 (kappa) and IgG3 (lambda); No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of Mouse IgG3 monoclonal antibody, clone RM218 (Cat# MAB12770) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IgG3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG3.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.005 - 0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5 - 2 ug/mL)
Immunohistochemistry (0.5 - 2 ug/mL)
Immunoprecipitation
Western Blot (0.1 - 0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG3 monoclonal antibody, clone RM218 (Cat # MAB12770) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both mouse IgG3 (kappa) and IgG3 (lambda); No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of Mouse IgG3 monoclonal antibody, clone RM218 (Cat# MAB12770) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — AI324046
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