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Last updated: 2016/11/27

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Mouse IgG2a (kappa) monoclonal antibody, clone RM107

  • Catalog # : MAB12767
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against native mouse IgG2a (kappa).
  • Immunogen:
  • Native purified IgG from mouse serum.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody reacts to the Fab region of mouse IgG2a. No cross reactivity with mouse IgG2a, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.005 - 0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5 - 2 ug/mL)
    Immunohistochemistry (0.5 - 2 ug/mL)
    Immunoprecipitation
    Western Blot (0.1 - 0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2a (kappa) (20 ng/lane) with Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat # MAB12767) under 0.2 ug/mL working concentration. This antibody only reacts to nonreduced mouse IgG2a (kappa).
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat # MAB12767) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fab region of mouse IgG2a (kappa); no cross reactivity with IgG2a (lambda), IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • Igh-1a
  • Gene Alias:
  • 1810060O09Rik,IGHG2A,Igh-1,MGC102662,MGC118125,MGC18977
  • Gene Description:
  • immunoglobulin heavy chain 1a (serum IgG2a)
  • Other Designations:
  • -
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