Mouse IgG2a (kappa) monoclonal antibody, clone RM107
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2a (kappa) (20 ng/lane) with Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat # MAB12767) under 0.2 ug/mL working concentration. This antibody only reacts to nonreduced mouse IgG2a (kappa).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat # MAB12767) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fab region of mouse IgG2a (kappa); no cross reactivity with IgG2a (lambda), IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG2aκ. The plate was coated with different amounts of mouse IgG2aκ. A serial dilution of Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat# MAB12767) was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IgG2a (kappa).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to the Fab region of mouse IgG2a. No cross reactivity with mouse IgG2a, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.005 - 0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5 - 2 ug/mL)
Immunohistochemistry (0.5 - 2 ug/mL)
Immunoprecipitation
Western Blot (0.1 - 0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2a (kappa) (20 ng/lane) with Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat # MAB12767) under 0.2 ug/mL working concentration. This antibody only reacts to nonreduced mouse IgG2a (kappa).Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat # MAB12767) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fab region of mouse IgG2a (kappa); no cross reactivity with IgG2a (lambda), IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG2aκ. The plate was coated with different amounts of mouse IgG2aκ. A serial dilution of Mouse IgG2a (kappa) monoclonal antibody, clone RM107 (Cat# MAB12767) was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — Igh-1a
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