Rabbit monoclonal antibody raised against native mouse IgG1.
Native purified IgG from mouse serum.
This antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG.
Protein A affinity purification
ELISA (0.005 - 0.2 ug/mL) Flow Cytometry Immunocytochemistry (0.5 - 2 ug/mL) Immunohistochemistry (0.5 - 2 ug/mL) Immunoprecipitation Western Blot (0.1 - 0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG1 (20 ng/lane) with Mouse IgG1 monoclonal antibody, clone RM106 (Cat # MAB12766) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced mouse IgG1 (~150 kDa) stronger than the reduced gamma 1 form (~50 kDa).
ELISA analysis of Mouse IgG1 monoclonal antibody, clone RM106 (Cat # MAB12766) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1; slightly cross reacts to IgG2b; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.