Rabbit monoclonal antibody raised against native mouse IgG.
Native purified IgG from mouse serum.
This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
Protein A affinity purification
ELISA (0.005 - 0.2 ug/mL) Flow Cytometry Immunocytochemistry (0.5 - 2 ug/mL) Immunohistochemistry (0.5 - 2 ug/mL) Immunoprecipitation Western Blot (0.1 - 0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of nonreduced (-) and reduced (+) mouse immunoglobulins (20 ng/lane) with Mouse IgG monoclonal antibody, clone RM104 (Cat # MAB12765) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
ELISA analysis of Mouse IgG monoclonal antibody, clone RM104 (Cat # MAB12765) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.