Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110
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More Files
- More Functions
Western Blot
Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat # MAB12763) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (lambda).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat # MAB12763) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG1λ. The plate was coated with different amounts of mouse IgG1λ. A serial dilution of Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat# MAB12763) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse immunoglobulin lambda light chain.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgM lambda.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.005 - 0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5 - 2 ug/mL)
Immunohistochemistry (0.5 - 2 ug/mL)
Immunoprecipitation
Western Blot (0.1 - 0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of nonreduced (-) and reduced (+) mouse IgG1 (kappa) and IgG1 (lambda) (20 ng/lane) with Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat # MAB12763) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG1 (lambda).Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat # MAB12763) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG1λ. The plate was coated with different amounts of mouse IgG1λ. A serial dilution of Mouse immunoglobulin lambda light chain monoclonal antibody, clone RM110 (Cat# MAB12763) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — Igl-C1
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