MMP14 monoclonal antibody, clone 133CT15.10.5.1
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of A2058 cell lysate (35 ug) with MMP14 monoclonal antibody, clone 133CT15.10.5.1 (Cat # MAB12762).
Immunofluorescence
Immunofluorescent staining of HepG2 cell with MMP14 monoclonal antibody, clone 133CT15.10.5.1 (Cat # MAB12762) followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human MMP14.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 470-499 at C-terminus region of human MMP14.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Euglobin precipitation
Isotype
IgM
Recommend Usage
Immunofluorescence (1:10 - 1:50)
Western Blot (1:100 - 1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of A2058 cell lysate (35 ug) with MMP14 monoclonal antibody, clone 133CT15.10.5.1 (Cat # MAB12762).Immunofluorescence
Immunofluorescent staining of HepG2 cell with MMP14 monoclonal antibody, clone 133CT15.10.5.1 (Cat # MAB12762) followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue). -
Gene Info — MMP14
Entrez GeneID
4323Protein Accession#
P50281Gene Name
MMP14
Gene Alias
MMP-X1, MT1-MMP, MTMMP1
Gene Description
matrix metallopeptidase 14 (membrane-inserted)
Omim ID
600754Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq
Other Designations
matrix metalloproteinase 14|matrix metalloproteinase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type matrix metalloproteinase 1|membrane-type-1 matrix metalloproteinase
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Interactome
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Pathway
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Disease
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Publication Reference
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From Microspikes to Stress Fibers: Actin Remodeling in Breast Acini Drives Myosin II-Mediated Basement Membrane Invasion.
Julian Eschenbruch, Georg Dreissen, Ronald Springer, Jens Konrad, Rudolf Merkel, Bernd Hoffmann, Erik Noetzel.
Cells 2021 Aug; 10(8):1979.
Application：IF, Human, Acini, MCF-10A cells.
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From Microspikes to Stress Fibers: Actin Remodeling in Breast Acini Drives Myosin II-Mediated Basement Membrane Invasion.
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