APOA1 monoclonal antibody, clone AT1E12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate with APOA1 monoclonal antibody, clone AT1E12 (Cat # MAB12761) at Lane 1: 1:500 dilution, Lane 2: 1:1000 dilution and Lane 3: 1:5000 dilution followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by ECL detection system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with APOA1 monoclonal antibody, clone AT1E12 (Cat # MAB12761).
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Specification
Product Description
Mouse monoclonal antibody raised against human APOA1.
Immunogen
Recombinant protein corresponding to amino acids 25-267 of human APOA1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate with APOA1 monoclonal antibody, clone AT1E12 (Cat # MAB12761) at Lane 1: 1:500 dilution, Lane 2: 1:1000 dilution and Lane 3: 1:5000 dilution followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by ECL detection system.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with APOA1 monoclonal antibody, clone AT1E12 (Cat # MAB12761).Enzyme-linked Immunoabsorbent Assay
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Gene Info — APOA1
Entrez GeneID
335Gene Name
APOA1
Gene Alias
MGC117399
Gene Description
apolipoprotein A-I
Gene Ontology
HyperlinkGene Summary
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq
Other Designations
OTTHUMP00000069346|OTTHUMP00000069347|OTTHUMP00000069348|apolipoprotein A1
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Interactome
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Pathway
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Disease
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