Western blot analysis of HeLa cell lysate with NANS monoclonal antibody, clone AT1G6 (Cat # MAB12760) at Lane 1: 1:100 dilution, Lane 2: 1:1000 dilution and Lane 3: 1:3000 dilution followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by ECL detection system.
This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq