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Last updated: 2017/11/19

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PCNA monoclonal antibody, clone PC10 (3F81)

  • Catalog # : MAB12708
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human PCNA.
  • Immunogen:
  • Human PCNA
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • Flow Cytometry
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (20 ug/mL)
    Immunoprecipitation
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.2 (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human uterus with PCNA monoclonal antibody, clone PC10 (3F81) (Cat # MAB12708) at 20 ug/mL working concentration.
  • Immunoprecipitation
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Molt-4 cells with PCNA monoclonal antibody, clone PC10 (3F81) (Cat # MAB12708) (colored histogram) and an isotype control mouse IgG2a, kappa (open histogram) followed by FITC-conjugated anti-mouse IgG as the secondary antibody.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 5111
  • Gene Name:
  • PCNA
  • Gene Alias:
  • MGC8367
  • Gene Description:
  • proliferating cell nuclear antigen
  • Gene Summary:
  • The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
  • Other Designations:
  • DNA polymerase delta auxiliary protein,OTTHUMP00000030189,OTTHUMP00000030190,cyclin
  • Interactome
  • Interactome
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