ACTA1 monoclonal antibody, clone 3B3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against human ACTA1.
Immunogen
A synthetic peptide corresponding to N-terminus region of human ACTA1.
Sequence
DEDETTALVC
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA
Immunohistochemistry (Frozen sections) (1:100-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skeletal muscle with ACTA1 monoclonal antibody, clone 3B3 (Cat # MAB12668) at 10 ug/mL working concentration.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACTA1
Entrez GeneID
58Gene Name
ACTA1
Gene Alias
ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Gene Description
actin, alpha 1, skeletal muscle
Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq
Other Designations
OTTHUMP00000036123|alpha skeletal muscle actin
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Interactome
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Disease
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