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VIM monoclonal antibody, clone 23H2

  • Catalog # : MAB12665
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human VIM.
  • Immunogen:
  • Recombinant protein corresponding to human VIM.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Purification:
  • Ammonium sulfate precipitation
  • Isotype:
  • IgG3, kappa
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:400)
    Immunoprecipitation
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In HEPES, 0.15 M NaCl (50% glycerol, 0.01% BSA, 0.03% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: RD2 cell lysate, Lane 2: C6 cell lysate and Lane 3: HeLa cell lysate with VIM monoclonal antibody, clone 23H2 (Cat # MAB12665).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human spleen with VIM monoclonal antibody, clone 23H2 (Cat # MAB12665) at 1:400 dilution.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 7431
  • Gene Name:
  • VIM
  • Gene Alias:
  • FLJ36605
  • Gene Description:
  • vimentin
  • Gene Summary:
  • This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
  • Other Designations:
  • OTTHUMP00000019224
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