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CENPF monoclonal antibody, clone 14C10 1D8

  • Catalog # : MAB12563
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human CENPF.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1759-2093 of human CENPF.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Immunofluorescence
    Immunohistochemistry (Frozen sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
    Immunoprecipitation
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2.
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human spleen with CENPF monoclonal antibody, clone 14C10 1D8 (Cat # MAB12563) at 10 ug/mL working concentration.
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • Immunoprecipitation
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1063
  • Gene Name:
  • CENPF
  • Gene Alias:
  • CENF,PRO1779,hcp-1
  • Gene Description:
  • centromere protein F, 350/400ka (mitosin)
  • Gene Summary:
  • This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq
  • Other Designations:
  • AH antigen,CENP-F kinetochore protein,OTTHUMP00000035028,cell-cycle-dependent 350K nuclear protein,centromere protein F,mitosin
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