Mouse monoclonal antibody raised against human MCL1.
Recombinant protein corresponding to human MCL1.
Protein G purification
ELISA (1:10000) Immunohistochemistry (Frozen sections) (1:200-1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000) Western Blot (1:200-1:1000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.03% sodium azide).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: HeLa cell line lysate, Lane 2: BCBL-1 cell line lysate, Lane 3: Jurkat cell line lysate and Lane 4: HL60 MG cell line lysate with MCL1 monoclonal antibody, clone 8C6 + 8C6D4B1 (Cat # MAB12518) at 1:200-1:1000 dilution.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human spleen with MCL1 monoclonal antibody, clone 8C6 + 8C6D4B1 (Cat # MAB12518) at 1:200-1:1000 dilution.
Immunofluorescent staining of HepG2 cells with MCL1 monoclonal antibody, clone 8C6 + 8C6D4B1 (Cat # MAB12518) (green) at 1:200-1:1000 dilution. Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. [provided by RefSeq