TAF15 monoclonal antibody, clone 4D71
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human TAF15.
Immunogen
Recombinant protein corresponding to full length human TAF15.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Immunofluorescence (1:500-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis tissue (A) and human colon tissue (B) using TAF15 monoclonal antibody, clone 4D71 (Cat # MAB12500) under 10 ug/mL working concentration.Immunofluorescence
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Gene Info — TAF15
Entrez GeneID
8148Protein Accession#
Q92804Gene Name
TAF15
Gene Alias
Npl3, RBP56, TAF2N, TAFII68, hTAFII68
Gene Description
TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Omim ID
601574Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
RBP56/CSMF fusion|RNA-binding protein 56|TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68 kD|TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56)|TATA box-binding protein-associ
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Interactome
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Disease
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