IL3RA monoclonal antibody, clone 10B8E7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293T cell lysate, Lane 2: IL3RA (a.a.: 200-305)-hIgGFc transfected HEK293T cell lysate using IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue (A) and human kidney tissue (B) using IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487) under 10 ug/mL working concentration.
Immunofluorescence
Immunofluorescent staining of HeLa cells using IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487) at 1:10000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human IL3RA.
Immunogen
Recombinant protein corresponding to amino acids 200-305 of human IL3RA.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry (1:200-1:100)
Immunofluorescence
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293T cell lysate, Lane 2: IL3RA (a.a.: 200-305)-hIgGFc transfected HEK293T cell lysate using IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue (A) and human kidney tissue (B) using IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487) under 10 ug/mL working concentration.Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of HeLa cells using IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IL3RA monoclonal antibody, clone 10B8E7 (Cat # MAB12487) at 1:10000 dilution.Flow Cytometry
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Gene Info — IL3RA
Entrez GeneID
3563Protein Accession#
P26951Gene Name
IL3RA
Gene Alias
CD123, IL3R, IL3RAY, IL3RX, IL3RY, MGC34174, hIL-3Ra
Gene Description
interleukin 3 receptor, alpha (low affinity)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq
Other Designations
CD123 antigen|Interleukin-3 receptor|OTTHUMP00000022828|interleukin 3 receptor, alpha
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Interactome
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Pathway
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Disease
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