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RASSF1 monoclonal antibody, clone 8E4

  • Catalog # : MAB12486
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human RASSF1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-341 of human RASSF1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (1% BSA, 50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: HEK293T cells were transfected with the pCMV6-ENTRY control, Lane 2: HEK293T cells were transfected with pCMV6-ENTRY RASSF1 cDNA for 48 hrs using RASSF1 monoclonal antibody, clone 8E4 (Cat # MAB12486) at 1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis tissue with RASSF1 monoclonal antibody, clone 8E4 (Cat # MAB12486) under 10 ug/mL working concentration.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of COS7 cells were transfected with the pCMV6-ENTRY RASSF1 using RASSF1 monoclonal antibody, clone 8E4 (Cat # MAB12486) at 1:100 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cyotometry anlaysis of Jurkat cells using RASSF1 monoclonal antibody, clone 8E4 (Cat # MAB12486) at 1:100 dilution.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • RASSF1
  • Gene Alias:
  • 123F2,NORE2A,RASSF1A,RDA32,REH3P21
  • Gene Description:
  • Ras association (RalGDS/AF-6) domain family member 1
  • Gene Summary:
  • This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000195503,Ras association (RalGDS/AF-6) domain family 1,Ras association domain family 1,Ras association domain family protein 1,WUGSC:H_LUCA12.5,cardiac-specific ras association domain family 1 protein,pancreas-specific ras association domain fam
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