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Last updated: 2017/8/20

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GAD2 monoclonal antibody, clone N-GAD65

  • Catalog # : MAB12482
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial synthetic peptide of human GAD2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 4-22 at the N-terminus of human GAD2.
  • Sequence:
  • PGSGFWSFGSEDGSGDSEN
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
    Immunoprecipitation
    Radioimmunoassay
    Western Blot (1:100-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum tissue with GAD2 monoclonal antibody, clone N-GAD65 (Cat # MAB12482) under 5 ug/mL working concentration.
  • Immunoprecipitation
  • Radioimmunoassay
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • Radioimmunoassay
  • Gene Information
  • Entrez GeneID:
  • 2572
  • Gene Name:
  • GAD2
  • Gene Alias:
  • GAD65,MGC161605,MGC161607
  • Gene Description:
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
  • Gene Summary:
  • This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq
  • Other Designations:
  • Glutamate decarboxylase-2 (pancreas),OTTHUMP00000019335,OTTHUMP00000019336,glutamate decarboxylase 2,glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
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