Mouse monoclonal antibody raised against partial recombinant human ITGB4.
Recombinant protein corresponding to amino acids 1619-1822 of human ITGB4.
ELISA (1:10000) Flow Cytometry (1:200-1:400) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: A549 cell lysate, Lane 2: A431 cell lysate, Lane 3: MCF-7 cell lysate, Lane 4: SW620 cell lysate using ITGB4 monoclonal antibody, clone 10B10D5 (Cat # MAB12480) at 1:500-1:2000 dilution.
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq