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POR monoclonal antibody, clone 6C4

  • Catalog # : MAB12467
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human POR.
  • Immunogen:
  • Recombinant protein corresponding to full length of human POR.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (1:100)
    Immunofluorescence (1:50-1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (1% BSA, 50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: HEK293T cells were transfected with the pCMV6-ENTRY control, Lane 2: HEK293T cells were transfected with pCMV6-ENTRY POR cDNA for 48 hrs using POR monoclonal antibody, clone 6C4 (Cat # MAB12467) at 1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue with POR monoclonal antibody, clone 6C4 (Cat # MAB12467) at 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cell lysate (A) and COS7 cells transfected by pCMV6-ENTRY POR (B) using POR monoclonal antibody, clone 6C4 (Cat # MAB12467) at 1:50-1:100 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow Cyotometry analysis of HeLa cell (A) and Jurkat cell (B) with POR monoclonal antibody, clone 6C4 (Cat # MAB12467) at 1:100 dilution.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5447
  • Gene Name:
  • POR
  • Gene Alias:
  • CPR,CYPOR,DKFZp686G04235,FLJ26468,P450R
  • Gene Description:
  • P450 (cytochrome) oxidoreductase
  • Gene Summary:
  • This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq
  • Other Designations:
  • NADPH-dependent cytochrome P450 reductase,cytochrome P450 reductase
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