Product Browser

Last updated: 2016/11/27

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

PSAP monoclonal antibody, clone 4D5F4

  • Catalog # : MAB12466
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human PSAP.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 325-524 of human PSAP.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry (1:50-1:500)
    Immunofluorescence (1:500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% protein stabilizer, 0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: HEK293 cells, Lane 2: PSAP (aa: 325-524)-hIgGFc transfected HEK293 cells with PSAP monoclonal antibody, clone 4D5F4 (Cat # MAB12466) at 1:500 - 1:2000 dilution.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of human PSAP recombinant protein (expected MW is 47.8 kDa) with PSAP monoclonal antibody, clone 4D5F4 (Cat # MAB12466) at 1:500-1:2000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney tissue with PSAP monoclonal antibody, clone 4D5F4 (Cat # MAB12466) under 5 ug/mL working concentration.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HepG2 cells with PSAP monoclonal antibody, clone 4D5F4 (Cat # MAB12466) at 1:500 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA analysis of PSAP monoclonal antibody, clone 4D5F42 (Cat # MAB12466) at 1:10000 dilution.
    Black: Control Antigen (100 ng); Purple: Antigen (10 ng); Blue: Antigen (50 ng); Red: Antigen (100 ng).
  • Flow Cytometry
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 5660
  • Gene Name:
  • PSAP
  • Gene Alias:
  • FLJ00245,GLBA,MGC110993,SAP1
  • Gene Description:
  • prosaposin
  • Gene Summary:
  • This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019776,sphingolipid activator protein-1
  • Gene Pathway
  • RSS
  • YouTube
  • Linkedin
  • Facebook