Mouse monoclonal antibody raised against partial recombinant human PSAP.
Recombinant protein corresponding to amino acids 325-524 of human PSAP.
Protein G purification
ELISA (1:10000) Flow Cytometry (1:200-1:400) Immunocytochemistry (1:50-1:500) Immunofluorescence (1:500) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In PBS (0.05% protein stabilizer, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney tissue with PSAP monoclonal antibody, clone 4D5F4 (Cat # MAB12466) under 5 ug/mL working concentration.
Immunofluorescent staining of HepG2 cells with PSAP monoclonal antibody, clone 4D5F4 (Cat # MAB12466) at 1:500 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq