Mouse monoclonal antibody raised against partial recombinant human KRT5.
Recombinant protein corresponding to amino acids 158-272 of human KRT5.
Human, Monkey, Mouse
Protein G purification
ELISA (1:10000) Flow Cytometry (1:200-1:400) Immunocytochemistry (1:200-1:1000) Immunofluorescence (1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In PBS (0.05% protein stabilizer, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: A431 cells, Lane 2: MCF-7 cell, Lane 3: HeLa cells, Lane 4: HepG2 cells, Lane 5: 3T3-L1 cells, Lane 6: COS-7 cells with KRT5 monoclonal antibody, clone 2C2 (Cat # MAB12460) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human CK5 (aa: 158-272) recombinant protein (expected MW is 33.3 kDa) with KRT5 monoclonal antibody, clone 2C2 (Cat # MAB12460) at 1:500-1:2000 dilution.
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq
58 kda cytokeratin,epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types,keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types),keratin, type II cytoskeletal 5