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ACAT1 monoclonal antibody, clone AT2C5

  • Catalog # : MAB12459
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human ACAT1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 34-427 of human ACAT1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HepG2 cells, Lane 2: HeLa cells with ACAT1 monoclonal antibody, clone AT2C5 (Cat # MAB12459) at 1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue with ACAT1 monoclonal antibody, clone AT2C5 (Cat # MAB12459) under 10 ug/mL working concentration.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 38
  • Gene Name:
  • ACAT1
  • Gene Alias:
  • ACAT,MAT,T2,THIL
  • Gene Description:
  • acetyl-Coenzyme A acetyltransferase 1
  • Gene Summary:
  • This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq
  • Other Designations:
  • acetoacetyl Coenzyme A thiolase
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