ACAT1 monoclonal antibody, clone AT2C5
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ACAT1.
Immunogen
Recombinant protein corresponding to amino acids 34-427 of human ACAT1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cells, Lane 2: HeLa cells with ACAT1 monoclonal antibody, clone AT2C5 (Cat # MAB12459) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue with ACAT1 monoclonal antibody, clone AT2C5 (Cat # MAB12459) under 10 ug/mL working concentration.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACAT1
Entrez GeneID
38Gene Name
ACAT1
Gene Alias
ACAT, MAT, T2, THIL
Gene Description
acetyl-Coenzyme A acetyltransferase 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq
Other Designations
acetoacetyl Coenzyme A thiolase
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Interactome
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Pathway
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Disease
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