Laminin monoclonal antibody, clone LAM-89
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against full length native of human laminin.
Immunogen
Native purified human laminin.
Host
Mouse
Reactivity
Cat, Human, Pig
Form
Lyophilized
Isotype
IgG1
Recommend Usage
Immunohistochemistry (0.5-1 ug/mL)
Western Blot (0.25-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from ascites fluid (1.2 % sodium acetate, 2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — LAMA2
Entrez GeneID
3908Gene Name
LAMA2
Gene Alias
LAMM
Gene Description
laminin, alpha 2
Gene Ontology
HyperlinkGene Summary
Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017189|laminin M|laminin alpha 2 subunit|merosin heavy chain
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Gene Info — LAMB1
Entrez GeneID
3912Gene Name
LAMB1
Gene Alias
CLM, MGC142015
Gene Description
laminin, beta 1
Omim ID
150240Gene Ontology
HyperlinkGene Summary
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq
Other Designations
cutis laxa with marfanoid phenotype
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Gene Info — LAMB2
Entrez GeneID
3913Gene Name
LAMB2
Gene Alias
LAMS
Gene Description
laminin, beta 2 (laminin S)
Gene Ontology
HyperlinkGene Summary
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region. However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron, which does not change the protein sequence, results in a greater abundance of the unspliced form of the transcript than the spliced form. The full-length nature of the spliced transcript is not known. [provided by RefSeq
Other Designations
laminin S|laminin, beta 2
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Gene Info — LAMA1
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Interactome
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Pathway
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Disease
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Publication Reference
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Malignant Peripheral Nerve Sheath Tumor in Children: A Clinicopathologic and Molecular Study with Parallels to the Adult Counterpart.
Narasimhan P Agaram, Leonard H Wexler, Ping Chi, Cristina R Antonescu.
Genes, Chromosomes & Cancer 2023 Mar; 62(3):131.
Application:IHC, Human, Human peripheral nerve sheath tumor.
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Malignant Peripheral Nerve Sheath Tumor in Children: A Clinicopathologic and Molecular Study with Parallels to the Adult Counterpart.
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