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Last updated: 2017/1/15

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Mouse Anti-human IgE secondary antibody, clone 4F7

  • Catalog # : MAB12445
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human IgE.
  • Immunogen:
  • Human IgE whole molecule.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This product is an IgG2a kappa antibody that was purified from tissue culture. It reacts with human IgE by indirect ELISA and does not react with IgM, IgA or IgG. > 90% pure as determined by page gel.
  • Form:
  • Lyophilized
  • Purification:
  • Antigen Affinity Purified
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • ELISA (2 ug/mL)
    Immunoprecipitation (2 ug/mg)
    Western Blot (0.05-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.2
  • Storage Instruction:
  • Store at 4°C.
    After reconstitution with 1 mL of reagent quality water, aliquots should be stored at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of Mouse Anti-human IgE secondary antibody, clone 4F7 (Cat# MAB12445) under 2 ug/mL working concentration.
  • Application Image
  • Western Blot
  • Immunoprecipitation
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