GFP Tag monoclonal antibody, clone 168AT1211
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of GFP recombinant protein reacted with GFP Tag monoclonal antibody (Cat # MAB12364) at 1:4000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag monoclonal antibody (Cat # MAB12364) at 1:50 dilution. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle.
Immunofluorescence
Immunofluorescence for GFP Tag Antibody (Cat # MAB12364). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes) at 1:200 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human GFP Tag.
Immunogen
Recombinant His fusion protein corresponding to human GFP.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Western Blot (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of GFP recombinant protein reacted with GFP Tag monoclonal antibody (Cat # MAB12364) at 1:4000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag monoclonal antibody (Cat # MAB12364) at 1:50 dilution. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle.Immunofluorescence
Immunofluorescence for GFP Tag Antibody (Cat # MAB12364). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes) at 1:200 dilution.
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