Immunohistochemical staining of formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag monoclonal antibody (Cat # MAB12364) at 1:50 dilution. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle.
Immunofluorescence for GFP Tag Antibody (Cat # MAB12364). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes) at 1:200 dilution.