RAC1 monoclonal antibody, clone 1301CT276.121.104
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell line lysates Lane 2: mouse NIH/3T3 cell line lysates Lane 3: rat C6 cell line lysates reacted with RAC1 monoclonal antibody (Cat # MAB12358) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human skin section reacted with RAC1 monoclonal antibody (Cat # MAB12358) at 1:25 dilution.
Flow Cytometry
Flow cytometric analysis of U-87 MG cells (green histogram) and an isotype control of mouse IgG2b (blue histogram) reacted with RAC1 monoclonal antibody (Cat # MAB12358) at 1:100 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human RAC1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human RAC1.
Host
Mouse
Reactivity
Human, Rat
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b
Recommend Usage
Flow Cytometry (1:100)
Immunohistochemistry (1:25)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell line lysates Lane 2: mouse NIH/3T3 cell line lysates Lane 3: rat C6 cell line lysates reacted with RAC1 monoclonal antibody (Cat # MAB12358) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human skin section reacted with RAC1 monoclonal antibody (Cat # MAB12358) at 1:25 dilution.Flow Cytometry
Flow cytometric analysis of U-87 MG cells (green histogram) and an isotype control of mouse IgG2b (blue histogram) reacted with RAC1 monoclonal antibody (Cat # MAB12358) at 1:100 dilution. -
Gene Info — RAC1
Entrez GeneID
5879Gene Name
RAC1
Gene Alias
MGC111543, MIG5, TC-25, p21-Rac1
Gene Description
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Omim ID
602048Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
migration-inducing gene 5|migration-inducing protein 5|ras-related C3 botulinum toxin substrate 1|ras-related C3 botulinum toxin substrate 1 isoform Rac1|ras-related C3 botulinum toxin substrate 1 isoform Rac1b|rho family, small GTP binding protein Rac1
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Interactome
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Pathway
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Disease
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Publication Reference
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The actin cytoskeleton is important for rotavirus internalization and RNA genome replication.
Trejo-Cerro O, Aguilar-Hernández N, Silva-Ayala D, López S, Arias CF.
Virus Research 2019 Jan; 263:27.
Application：WB-Tr, Monkey, MA104 cells.
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The actin cytoskeleton is important for rotavirus internalization and RNA genome replication.
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