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CAPN2 monoclonal antibody, clone 1381CT669.7.66.71

  • Catalog # : MAB12357
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of human CAPN2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to human CAPN2.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • Flow Cytometry (1:100)
    Immunohistochemistry (1:25)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: A549 cell line lysates Lane 2: A431 cell line lysates Lane 3: SK-BR-3 cell line lysates reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human stomach section reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:25 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of U-87 MG cells (green histogram) and an isotype control of mouse IgG2b (blue histogram) reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 824
  • Gene Name:
  • CAPN2
  • Gene Alias:
  • CANP2,CANPL2,CANPml,FLJ39928,mCANP
  • Gene Description:
  • calpain 2, (m/II) large subunit
  • Gene Summary:
  • The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • M-calpain,OTTHUMP00000035497,calcium-activated neutral proteinase,calpain 2, large [catalytic] subunit,calpain 2, large subunit,calpain M-type,calpain, large polypeptide L2,millimolar-calpain
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