CAPN2 monoclonal antibody, clone 1381CT669.7.66.71
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell line lysates Lane 2: A431 cell line lysates Lane 3: SK-BR-3 cell line lysates reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human stomach section reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:25 dilution.
Flow Cytometry
Flow cytometric analysis of U-87 MG cells (green histogram) and an isotype control of mouse IgG2b (blue histogram) reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:100 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human CAPN2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human CAPN2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b
Recommend Usage
Flow Cytometry (1:100)
Immunohistochemistry (1:25)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell line lysates Lane 2: A431 cell line lysates Lane 3: SK-BR-3 cell line lysates reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human stomach section reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:25 dilution.Flow Cytometry
Flow cytometric analysis of U-87 MG cells (green histogram) and an isotype control of mouse IgG2b (blue histogram) reacted with CAPN2 monoclonal antibody (Cat # MAB12357) at 1:100 dilution. -
Gene Info — CAPN2
Entrez GeneID
824Gene Name
CAPN2
Gene Alias
CANP2, CANPL2, CANPml, FLJ39928, mCANP
Gene Description
calpain 2, (m/II) large subunit
Omim ID
114230Gene Ontology
HyperlinkGene Summary
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
M-calpain|OTTHUMP00000035497|calcium-activated neutral proteinase|calpain 2, large [catalytic] subunit|calpain 2, large subunit|calpain M-type|calpain, large polypeptide L2|millimolar-calpain
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Interactome
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Pathway
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Disease
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