RPS6 monoclonal antibody, clone 981CT2.1.1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293 cell line lysates reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain section reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:25 dilution.
Immunofluorescence
Immunofluorescent staining of Hela cells reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:25 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells (green histogram) and an isotype control of mouse IgG1 (blue histogram) reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:25 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human RPS6.
Immunogen
Recombinant protein corresponding to human RPS6.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA
Flow Cytometry (1:25)
Immunohistochemistry (1:25)
Immunofluorescence (1:25)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293 cell line lysates reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain section reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:25 dilution.Immunofluorescence
Immunofluorescent staining of Hela cells reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:25 dilution.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hela cells (green histogram) and an isotype control of mouse IgG1 (blue histogram) reacted with RPS6 monoclonal antibody (Cat # MAB12353) at 1:25 dilution. -
Gene Info — RPS6
Entrez GeneID
6194Protein Accession#
P62753Gene Name
RPS6
Gene Alias
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Gene Description
ribosomal protein S6
Omim ID
180460Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S6|OTTHUMP00000021120|phosphoprotein NP33
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Interactome
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Pathway
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