ENO1 monoclonal antibody, clone 786CT6.6.4
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More Files
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Western Blot
Western blot analysis of Lane 1: MCF-7 cell line lysates Lane 2: A431 cell line lysates Lane 3: Hela cell line lysates Lane 4: HepG2 cell line lysates Lane 5: mouse NIH/3T3 cell line lysates Lane 6: mouse stomach tissue lysates Lane 7: liver tissue lysates reacted with ENO1 monoclonal antibody (Cat # MAB12329) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human kidney section reacted with ENO1 monoclonal antibody (Cat # MAB12329) at 1:25 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human ENO1.
Immunogen
Recombinant His fusion protein corresponding to human ENO1.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG, kappa
Recommend Usage
Immunohistochemistry (1:25)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: MCF-7 cell line lysates Lane 2: A431 cell line lysates Lane 3: Hela cell line lysates Lane 4: HepG2 cell line lysates Lane 5: mouse NIH/3T3 cell line lysates Lane 6: mouse stomach tissue lysates Lane 7: liver tissue lysates reacted with ENO1 monoclonal antibody (Cat # MAB12329) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human kidney section reacted with ENO1 monoclonal antibody (Cat # MAB12329) at 1:25 dilution. -
Gene Info — ENO1
Entrez GeneID
2023Gene Name
ENO1
Gene Alias
ENO1L1, MBP-1, MPB1, NNE, PPH
Gene Description
enolase 1, (alpha)
Omim ID
172430Gene Ontology
HyperlinkGene Summary
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq
Other Designations
2-phospho-D-glycerate hydro-lyase|MYC promoter-binding protein 1|OTTHUMP00000001706|alpha enolase like 1|enolase 1|non-neural enolase|phosphopyruvate hydratase|tau-crystallin
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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