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Last updated: 2017/11/19

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SOX2 monoclonal antibody, clone 57CT23.3.4

  • Catalog # : MAB12319
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human SOX2.
  • Immunogen:
  • Recombinant protein corresponding to human SOX2.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (1:10-1:50)
    Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:100)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of NCCIT cell line lysates reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of A549 cell reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:100 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NCI-H460 cells (bottom histogram) and a negative control cell (top histogram) reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:10-1:50 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6657
  • Gene Name:
  • SOX2
  • Gene Alias:
  • ANOP3,MCOPS3,MGC2413
  • Gene Description:
  • SRY (sex determining region Y)-box 2
  • Gene Summary:
  • This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq
  • Other Designations:
  • SRY-related HMG-box gene 2,sex-determining region Y-box 2,transcription factor SOX2
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