SOX2 monoclonal antibody, clone 57CT23.3.4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NCCIT cell line lysates reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of A549 cell reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:100 dilution.
Flow Cytometry
Flow cytometric analysis of NCI-H460 cells (bottom histogram) and a negative control cell (top histogram) reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against human SOX2.
Immunogen
Recombinant protein corresponding to human SOX2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (1:10-1:50)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NCCIT cell line lysates reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of A549 cell reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:100 dilution.Flow Cytometry
Flow cytometric analysis of NCI-H460 cells (bottom histogram) and a negative control cell (top histogram) reacted with SOX2 monoclonal antibody (Cat # MAB12319) at 1:10-1:50 dilution. -
Gene Info — SOX2
Entrez GeneID
6657Gene Name
SOX2
Gene Alias
ANOP3, MCOPS3, MGC2413
Gene Description
SRY (sex determining region Y)-box 2
Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq
Other Designations
SRY-related HMG-box gene 2|sex-determining region Y-box 2|transcription factor SOX2
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Interactome
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Disease
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